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Fakultät
Botanik
Botanik I
Botanik I intern
T. Lamparter
Lehre/Teaching
Klone, Tabellen |
Prof. Dr. Tilman Lamparter, Karlsruhe Institute of Technology KIT
Prof. Dr. Tilman Lamparter Karlsruhe Institute of Technology Botanical Institute Kaiserstr. 2 - Gebäude 10.40 D-76131 Karlsruhe phone (+49)(0)721 608 45441 FAX (+49)(0)721 608 44193 e-mail tilman.lamparter @ kit.edu Born 1959 in Sindelfingen. Studying biology and plant physiology in Tübingen and Freiburg. 1992 research fellow at the Freie Universität (FU) Berlin (DFG research grant). 1992-1998, Research Fellow (Wissenschaftlicher Mitarbeiter) in the group of Prof. E. Hartmann, FU Berlin. 1996, 1999 and 2000, research visits Institute for Basic Biology, Okasaki, Japan (Prof. M. Wada), 1996 research visit University Leeds (Prof. Cove). 1998-2002, Project Leader (Akademischer Mitarbeiter) FU Berlin. January 2000, habilitation in Plant Physiology. 2002-2007 Project Leader (Oberassistent) FU Berlin. Since April 2007: Professor at the Institut für Botanik I, Universität Karlsruhe. Principal research interestsMy research interest is focused on a photoreceptor termed phytochrome. Many steps in plant development such as seed germination, flower induction, shade avoidance or de-etiolation are under phytochrome control. In my group we use bacterial phytochromes from e.g. Agrobacterium tumefaciens and Synechocystis PCC6803 as models for photoconversion. Upon light absorption phytochromes undergo typical spectral changes and protein conformational changes that trigger signal transduction. The molecular mechanisms behind these conformational changes are as yet unknown. We also use mosses, which stand at the base of land plant evolution, as models to study phytochrome action at the cellular level. With the help of mutants, we have identified specific phytochrome effects in these cells. In a future project it is planned to isolate microalgae for biomass production.Projects1. Biochemical studies on bacterial phytochromes, photoconversion2. Agrobacterium tumefaciens light responses 3. Moss phototropism and other phytochrome responses, chromophore biosynthesis, microinjection 4. Screening algae for biomass production ApproachesRecombinant protein expression, biochemical characterization, proteolysis, size exclusion chromatography, protein crosslinking, site directed mutagenesis, UV/vis spectroscopy, in vivo phytochrome measurements, protein crystallization, physiological assays, microinjection.Selected publicationsRui Yanga, Kaori Nishiyamab, Ayumi Kamiyab, Yutaka Ukajib, Katsuhiko Inomata, Tilman Lamparter (2012)Assembly of Synthetic Locked Phycocyanobilin Derivatives with Phytochrome in Vitro and in Vivo in Ceratodon purpureus and Arabidopsis. The Plant Cell klick here for articleOberpichler I, Rosen R, Rasouly A, Vugman M, Ron EZ, Lamparter T (2008) Light affects motility and infectivity of Agrobacterium tumefaciens. Environmental Microbiology, 10, 2020-2029 Noack S, Michael N, Rosen R, Lamparter T (2007) Protein conformational changes of Agrobacterium phytochrome Agp1 during chromophore assembly and photoconversion. Biochemistry, 46, 4164-4176 Inomata K, Noack S, Hammam MA, Khawn H, Kinoshita H, Murata Y, Michael N, Scheerer P, Krauss N, Lamparter T. (2006) Assembly of synthetic locked chromophores with Agrobacterium phytochromes Agp1 and Agp2. J. Biol. Chem. 281, 28162-28173. Lamparter T (2005) Photomorphogenesis of mosses. In: Photomorphogenesis in Plants, 3rd edition, ed. by E. Schäfer and F. Nagy Lamparter, T (2004) Evolution of cyanobacterial and plant phytochromes. FEBS Letters 573, 1-5 Lamparter T, Michael N, Mittmann F, Esteban E (2002) Phytochrome from Agrobacterium tumefaciens has unusual spectral properties and reveals a new chromophore attachment site. PNAS 99, 11628-11633.
Lehre / teaching Good practice email rules Gremien Biologie Chemie am KIT Tilman Lamparter |