Botanik - Molekulare Zellbiologie


 Botanisches Institut              Exercise - Cytoskeleton

AG Nick   

Background Material

A well made introduction into the logics of pattern formation and reaction-inhibitor models on Botanik Online

A model of phyllotaxis based on mutual competition of primordia for free auxin from the Kuhlemeier-lab

Short review on the actin-auxin oscillator Nick 2010

Short review on plant patterning and cellular noise Nick 2007

chapter on xylem vessel development in the Illuminated Plant Cell

 

 


1. In multicellular animals, the fertilised zygote (egg cell) is the only cell with totipotency. Do the cells of the soma contain still the entire set of genes? How can this be tested experimentally?


2. How would the stomata pattern look like in an Arabidopsis thaliana mutant overexpressing the factor too many mouths (tmm)?


3. When leaves of Arabidopsis thaliana are treated with the phytotropin NPA (an inhibitor of auxin efflux), the following pattern is observed (see figure, J, from Mattson et al. 1999). Can you explain this result on the basis of the auxin-canalisation model?

 

 

 

 

 

 

 


4. You want to make an ethanolic stock solution for the natural auxin indolyl-3-acetic acid (IAA, MW 181). The working concentrations are in the range of 1-50 µM, the solvent ethanol should not exceed 1 % to avoid cell damage. You decide to make 10 ml of a stock solution of 1 mM. What quantity of solid IAA is needed?


5. What would happen, if all cells of a mustard cotyledon would have identical response thresholds?


 

 

 

 

 


© 2012 Peter Nick, Botanisches Institut 1, alle Rechte vorbehalten
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