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General on GFP
Martin Chalfie's
Nobel Prize Speech on GFP
simple DNatube movie on the
discovery of GFP and the significance of this discovery
Youtube-movie zur Anwendung von
GFP, um
neuronale Stammzellen in Mäusen nachzuweisen
History, application, development and
evolution of fluorescent proteinen ("Biologie
in unserer Zeit")
Aequorin
The classical paper by the Trewavas
group on aequorin plants (you need to be logged in via vpn to get
the
pdf)
New approach to detect protein
translocation to the membrane based on the Aequorin reporter (Giorgi
et al. 2011)
Photoactivable GFP
Publication of
Mathur et al. 2010 on
the use of the photoactivable Dendra protein
Publication of
Baker et al. 2010 on
the use of the photoactivable Dendra protein
Publication by
Adam et al. 2008 on
mIRIS
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1. You want
to design a GFP mutant, where the spectrum is shifted towards longer
wavelength. Which strategy would you use?
2. For what
application did Martin Chalfie plant to use GFP? What alternative
approaches existed at that time? Why was he initially sceptical, whether
the GFP approach would work. You have to read for this his Nobel Prize
Speech.
3. What happens, when you irradiate
mIRIS with near UV and then with green?
o
it is green fluorescent
o
it is red fluorescent
o
it is blue fluorescent
o it
is not fluorescent at all
4. How can
actin polarity determined by the help of EosFP (you have to
read Mathur et al. 2010 for answering this).
5. Describe
an approach
to follow integration of proteins into the membrane in real time using
the aequorin reporter
© 2012 Peter Nick, Botanisches Institut 1, alle
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Letzte Änderung Samstag, 28. Januar 2012
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