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Flash-animation on the patch clamp technique
Background on the laser-assisted
thermal microinjection.
Higashiyama et al. 2008
Description of the use of FACS with protoplast from fluorescently
tagged root-tissue marker lines.
Publication on our work
demonstrating the use of caged auxin on the subcellular level
Kusaka et
al. 2009.
Laser-capture microdissection to
identify defence genes
Chandran et al. 2010
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1.
How can the following molecules pass
the plasma membrane barreer?
water, glucose, protons,
insulin, the peptide BP100
2. You have isolated a
membrane protein, and you suspect that this protein might be an
aquaporin. How can you test this hypothesis experimentally?
3.
Most
signals are perceived by receptors at the plasma membrane, but later
result in gene activation. Explain by which mechanisms the information
is transmitted between these two events.
4. You want
to find out, which genes are upregulated in the colonised host cells of
Grapevine affected by Grapevine Downy Mildew. Propose a strategy.
5. Explain,
why caged auxin has to be designed such that it is esterase-insensitive
and how this was achieved. Background
Kusaka et al. 2009.
© 2012 Peter Nick, Botanisches Institut 1, alle
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Letzte Änderung Samstag, 28. Januar 2012 |
